hNR1I2

通过同源重组，将小鼠Nr1i2基因进行人源化修饰。

Fig1. Detection of NR1I2 expression by RT-PCR. 

Wild type: only one band at 228 bp with primers F2/R2(mNr1i2);

Homozygous: only one band at 172 bp with primers F1/R1(hNR1I2); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig.2 Detection of NR1I2 expression in duodenum and large Intestine by RT-PCR. Mouse Nr1i2 mRNA (217 bp) was detectable only in wild-type C57BL/6 mice. Human NR1I2 mRNA (361 bp) was detectable only in homozygous hNR1I2 knockin mice but not in wild-type mice. Duodenum and large Intestine RNA was extracted from 6-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hNR1I2 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.3 Detection of NR1I2 expression in hNR1I2 mice by WB. NR1I2 was detectable in liver and colon from both WT C57BL/6 and HO hNR1I2 mice, as the antibody was cross-reactived between human and mouse NR1I2. The liver and colon tissue lysates were collected from 7-week-old female wild-type C57BL/6 mice and 6-week-old female homozygous hNR1I2 mice, and then analyzed by western blot. 

Abbr. HO, homozygous; WT, wild type.