hAPOE2

Fig.1 Detection of APOE expression in brain and liver by RT-PCR. Mouse Apoe mRNA (193 bp) was detectable only in wild-type C57BL/6 mice but not in hAPOE knockin mice. Human APOE mRNA (207 bp) was detectable only in homozygous hAPOE2 knockin mice but not in wild-type mice. Tissue RNA was extracted from wild-type C57BL/6 mice (WT) and homozygous hAPOE2 knockin mice (HO) (n=5 per group, male, 7 weeks old), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.

Fig.2 Detection of human APOE(A) and mouse APOE(B) expression in serum of WT C57BL/6 mice and HO hAPOE2 mice by ELISA.  Mouse APOE was exclusively detectable in wild-type C57BL/6 mice but not in hAPOE2 knockin mice, and human APOE was exclusively detectable in homozygous hAPOE2 knockin mice. Serum samples were collected from wild-type C57BL/6 mice and HO hAPOE2 mice (n=5 per group, male, 7 weeks old), and analyzed by ELISA with an anti-mAPOE ELISA kit and an anti-hAPOE ELISA kit. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human APOE(A) and mouse APOE(B) expression in brain of WT C57BL/6 mice and HO hAPOE2 mice by ELISA.  Mouse APOE was exclusively detectable in the brain of WT C57BL/6 mice but absent in hAPOE2 knockin mice. Human APOE was positively detectable in the brain of homozygous hAPOE2 knockin mice, although the kit exhibited non-specific detection in brain lysates of WT mice. Brain tissues lysates were collected from WT C57BL/6 mice and HO hAPOE2 mice (n=5 per group, male, 7 weeks old), and analyzed by ELISA with an anti-mAPOE ELISA kit and an anti-hAPOE ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D., not detectable.

Fig.4 Detection of human APOE(A) and mouse APOE(B) expression in liver of WT C57BL/6 mice and HO hAPOE2 mice by ELISA. Mouse APOE was exclusively detectable in the liver of WT C57BL/6 mice but absent in hAPOE2 knockin mice. Human APOE was positively detectable in the liver of homozygous hAPOE2 knockin mice, although the kit exhibited non-specific detection in liver lysates of WT mice. Liver tissues lysates were collected from WT C57BL/6 mice and HO hAPOE2 mice (n=5 per group, male, 7 weeks old), and analyzed by ELISA with an anti-mAPOE ELISA kit and an anti-hAPOE ELISA kit. 

Abbr. HO, homozygous; WT, wild type; N.D., not detectable.

Fig.3 Blood lipid profiles of hAPOE2 knockin mice. Homozygous hAPOE2 mice exhibited significantly elevated serum TG, T-CHO and LDL-C levels compared to wild-type C57BL/6 mice, indicating the lipid dysmetabolism in hAPOE2 knockin mice. Serum samples were collected from wild-type C57BL/6 mice and HO hAPOE2 mice (n=5 per group, male, 7 weeks old) to measure levels of (A) triglycerides (TG), (B) total cholesterol (T-CHO), (C) high-density lipoprotein cholesterol (HDL-C) and (D) low-density lipoprotein cholesterol (LDL-C (n=5 per group, Mean ± SEM, t-test, *p < 0.05, ***p < 0.001, ****p < 0.0001, ns, no significance). 

Abbr. HO, homozygous; WT, wild type.