Ctsk-2A-Cre

品系全名

C57BL/6JSmo-Ctskem1(2A-Cre-Wpre-pA)Smoc

目录号

NM-KI-190019

品系状态

活体

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基因信息

基因名
Ctsk

品系描述

将2A-Cre-Wpre-polyA共表达结构插入小鼠内源Ctsk基因3'UTR之前。
应用领域:Cre工具鼠,破骨细胞特异性

验证数据


ctsk-cre-1.png

Fig.1 荧光检测小鼠胚胎(E16.5)tdTomato表达。 Ctsk-(2A-Cre-WPRE-pA)小鼠与Rosa26-LSL-tdTomato小鼠交配,取E16.5胚胎,进行荧光显微镜拍照。结果显示:Cre可在胚胎的全身皮肤、耳朵、眼眶、大脑、四肢骨关节等部位表达。

ctsk-cre-2.png


Fig.2 荧光检测 新生鼠(P7)tdTomato表达。Ctsk-(2A-Cre-Wpre-pA)小鼠与Rosa26-LSL-tdTomato小鼠交配,取第七天双阳性新生鼠进行荧光显微镜拍照。结果显示在皮肤(较胚胎期弱)、骨骼部位有红色荧光,心肺、肝肾、肠胃等内脏没有红色荧光表达。


发表文献 1篇

1. Ubiquitin-Specific Protease 34 Inhibits Osteoclast Differentiation by Regulating NF-κB Signaling
发表年份:2020 来源杂志:JOURNAL OF BONE AND MINERAL RESEARCH

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