Gpr65-IRES-Cre

品系全名

C57BL/6JSmo-Gpr65em1(IRES-iCre)Smoc

目录号

NM-KI-200135

品系状态

精子冻存

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基因信息

基因名
Gpr65

品系描述

将IRES-iCre插入到小鼠Gpr65基因终止密码子处。
应用领域:Cre工具鼠;G 蛋白偶联受体65(GPR65)支配肠绒毛或胃粘膜感受营养物质。当该小鼠与含有loxP侧翼序列的小鼠交配时,Cre介导的重组将导致后代表达Cre的细胞中floxed 序列的缺失。

验证数据

image.png

图 1 Gpr65Cre/+; Rosa26tdTomato/+ 小鼠肠道中tdTomato的表达情况。

Gpr65Cre/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠大肠和小肠中检测到tdTomato的表达。

image.png

图 2 Gpr65Cre/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况。TdTomato的表达可广泛表达在垂体、心脏、肺、肝、胃、唾液腺、肾、胰腺、大肠、肌肉、皮肤、睾丸和附睾中。个别细胞表达的组织有:胸腺和脾脏。不表达的组织有:棕色脂肪组织。(更多详细信息请联系我司技术顾问。)



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