hTSLP(BALB/c)

Fig.1 Detection of TSLP expression in bladder by RT-PCR. Mouse Tslp mRNA (125 bp) was exclusively detectable in wild-type BALB/c mice but not in homozygous hTSLP(BALB/c) knock-in mice. Human TSLP mRNA (160 bp) was detectable only in hTSLP(BALB/c) knock-in mice but not in wild-type BALB/c mice. Bladder RNA was extracted from 9-week-old female wild-type BALB/c (n=3) and 9-week-old female homozygous hTSLP(BALB/c) knock-in mice (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human TSLP expression in the ear of MC903-induced WT BALB/c mice and hTSLP(BALB/c) mice by ELISA. Human TSLP was detectable in ears from homozygous hTSLP(BALB/c) knockin mice under the induction by MC903, with the highest expression level at about 4 days post-induction. Male, 6-week-old WT BALB/c mice and homozygous hTSLP(BALB/c) mice were treated with MC903 (3 nmol in 20 μL ethanol) on one ear once daily. Ear tissues were collected from mice at 0, 2, 4, and 7 days post-treatment, homogenized, and analyzed by ELISA with an anti-hTSLP ELISA kit (n=3/group, Mean±SEM, One-way ANOVA, *p<0.05, ****p<0.0001). 

Abbr.  HO, homozygous; WT, wild type.

Fig.3 Detection of mouse TSLP expression in the ear of MC903-induced WT BALB/c mice and hTSLP(BALB/c) mice by ELISA. Mouse TSLP was detectable in ears from WT BALB/c mice under the induction by MC903, with the highest expression level at about 4 days post-induction.

Male, 6-week-old WT BALB/c mice and homozygous hTSLP(BALB/c) mice were treated with MC903 (3 nmol in 20 μL ethanol) on one ear once daily. Ear tissues were collected from mice at 0, 2, 4, and 7 days post-treatment, homogenized, and analyzed by ELISA with an anti-mTSLP ELISA kit (n=2 or 3/group, Mean±SEM, One-way ANOVA, *p<0.05, ****p<0.0001). 

Abbr.  HO, homozygous; WT, wild type; N.D., not detectable.