Hand2-2A-EGFP-2A-CreERT2
品系全名
C57BL/6Smoc-Hand2em1(P2A-EGFP-T2A-CreERT2)Smoc
目录号
NM-KI-250149
品系状态
活体
基因信息
基因名
Hand2
品系描述
将P2A-EGFP-T2A-CreERT2插入到小鼠Hand2基因终止密码子处。
*使用本品系发表的文献需注明: Hand2-2A-EGFP-2A-CreERT2 mice (Cat. NO. NM-KI-250149) were purchased from Shanghai Model Organisms Center, Inc..
验证数据
Fig1. CreERT2 mediated recombination in heart of Hand2EGFP-2A-CreERT2/+; Rosa26tdTomato/+ mouse.
Fig2. Detection of tdTomato(red) and EGFP(green) in various tissues of Hand2EGFP-2A-CreERT2/+; Rosa26tdTomato/+ mouse.
Following tamoxifen induction, tdTomato expression was observed in the heart, liver, large intestine, small intestine, stomach, pancreas, and vagina.
EGFP expression was detected in the liver, large intestine, and small intestine.
(51-days old, female) (For more detailed information please contact our technical advisor.)
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Tamoxifen诱导Cre-ERT2小鼠 使用指南
Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。
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