hTLR8

Fig1. Detection of TLR8 expression in spleen, lung and thymus by RT-PCR. Wild type: only one band at 318 bp with primers F1/R1(mTlr8);Homoygous: only one band at 366 bp with primers F2/R2(hTLR8); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig 2. The representative picture of hepatomegaly(top), splenomegaly (bottom) in aged hTLR8 mice.

Fig 3. Histology of (A) spleen and (B) liver in aged hTLR8 mice. A prominent portal inflammation with fibrosis and bile duct hyperplasia in liver (A); Decreased cellularity in periarteriolar lymphoid sheath and the increase of histiocytes in the red pulp of spleen (B). 

Fig 4. The expression pattern of hTLR8 in myeloid cells (A, B); and cytokine analysis in serum of aged hTLR8 mice (C).

Fig 5. The dynamic change of (A) myeloid and (B) lymphocyte lineage in the spleen of hTLR8 mice; and (C) in vitro stimulation of splenocytes from wild type (WT) C57BL/6 and hTLR8 mice with hTLR8 agonist ORN-06

Fig 6. MC-38 tumor growth curve and tumor weight of individual mice from the 4 groups. One way ANOVA *, **, and *** refer to p<0.05, p<0.01, and p<0.001, respectively

Fig 7. Serum level of cytokine analysis by MSD and intracellular TNF-a staining by FACS in splenocytes.

Fig 8. Tumor infiltrated immune component analysis.

Fig 9. Pro-inflammatory cytokines in the tumor cell lysate. *Validation data are provided by Crownbio.