Il6-2A-DTR-2A-EGFP
品系全名
C57BL/6Smoc-Il6em1(P2A-DTR-T2A-EGFP-WPRE-pA)Smoc
目录号
NM-KI-242149
品系状态
活体
基因信息
基因名
IL6
品系描述
将P2A-DTR-T2A-EGFP-WPRE-pA插入到小鼠Il6基因终止密码子处。
验证数据
Fig.1 Detection of EGFP expression in blood macrophages of Il6-2A-DTR-2A-EGFP mice by FACS.
Il6-2A-DTR-2A-EGFP mice that received an intraperitoneal dose of LPS (5 mg/kg) and were sacrificed 2 h later exhibited a pronounced increase of EGFP+ signals in blood (n=2, male, 6 weeks old).
Abbr. HE, heterozygous; WT, wild type.
Fig.2 Detection of EGFP expression in bone marrow macrophages of Il6-2A-DTR-2A-EGFP mice by FACS.
Il6-2A-DTR-2A-EGFP mice that received an intraperitoneal dose of LPS (5 mg/kg) and were sacrificed 2 h later exhibited a pronounced increase of EGFP+ signals in bone marrow (n=2, male, 6 weeks old).
Abbr. HE, heterozygous; WT, wild type.
Fig.3 Detection of EGFP expression in spleen macrophages of Il6-2A-DTR-2A-EGFP mice by FACS.
Il6-2A-DTR-2A-EGFP mice that received an intraperitoneal dose of LPS (5 mg/kg) and were sacrificed 2 h later exhibited a pronounced increase of EGFP+ signals in spleen (n=2, male, 6 weeks old).
Abbr. HE, heterozygous; WT, wild type.
Fig.4 Detection of EGFP expression in liver macrophages of Il6-2A-DTR-2A-EGFP mice by FACS.
Il6-2A-DTR-2A-EGFP mice that received an intraperitoneal dose of LPS (5 mg/kg) and were sacrificed 2 h later exhibited a pronounced increase of EGFP+ signals in liver (n=2, male, 6 weeks old).
Abbr. HE, heterozygous; WT, wild type.
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