Apoe-KO/Nos3-KO

Fig.1 Detection of Apoe/Nos3 expression in mammary gland by RT-PCR. 

Wild type: one band at 987 bp with primers F1/R1(mApoe), one band at 113 bp with primers F2/R2(mNos3) and one band at 123 bp with primers F/R(mGapdh).

Homozygous: one band at ~150bp with primers F1/R1(mApoe) and one band at 123 bp with primers F/R(mGapdh).

Heterozygous: two bands at 987 bp/~150bp with primers F1/R1(mApoe) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HE, heterozygous; HO, homozygous; WT, wild type.

Fig.2 Detection of mouse APOE expression in Apoe-KO/Nos3-KO mice by WB. Various tissues lysates were collected from wild-type C57BL/6 mice (WT, male, 6 weeks old) and heterozygous (HE/HO, male, 6 weeks old) and homozygous Apoe-KO/Nos3-KO mice (HO/HO, female, 5 weeks old), and then analyzed by western blot with anti-APOE antibody (Abcam, ab183596). 20 μg of total proteins were loaded for western blotting analysis.