Htt-hHTT-Q145

Fig.1 Body weight and brain weight of WT C57BL/6 mice (n=3, 22-week-old; 1 male, 2 females) and Htt-hHTT-Q145 mice (n=3, 23-week-old females). The female heterozygous Htt-hHTT-Q145 mice exhibited significantly lower body weight than WT mice.

Abbr. HE, heterozygous; WT, wild type.

Fig.2 Detection of HTT expression in Htt-hHTT-Q145 mice by WB. HTT was detectable in the hippocampus, cortex, and cerebellum of both WT C57BL/6 and HE Htt-hHTT-Q145 mice, as the antibody cross-reacted with both human and mouse HTT. In tissues from WT mice, HTT protein appeared as a single band, whereas in HE mice, it appeared as two bands: one at the same position as WT HTT and another at a higher molecular weight corresponding to hHTT-Q145. The hippocampus, cortex and cerebellum tissue lysates were collected from WT C57BL/6 mice (n=3, 22-week-old; 1 male, 2 females) and Htt-hHTT-Q145 mice (n=3, 23-week-old females), and then analyzed by western blot. 

Abbr. HE, heterozygous; WT, wild type; PC, positive control, Hela cells.

Fig3. Detection of human HTT expression in WT C57BL/6 mice and Htt-hHTT-Q145 mice by IHC. Human HTT was not detectable in the cortex and corpus striatum of WT C57BL/6 mice, whereas it was clearly detectable in these same regions of heterozygous Htt-hHTT-Q145 mice. The brain was collected from 22-week-old WT C57BL/6 mice and 23-week-old heterozygous Htt-hHTT-Q145 mice to perform IHC staining. The black arrows indicate the hHTT-positive areas. Scale bars: 0.5 mm for panorama gram and 0.1 mm for cortex and corpus striatum images.

Abbr. WT, wild type. HE, heterozygous.