hTP53-R273H
品系全名
C57BL/6JSmo-Trp53tm1(hTP53-R273H)Smoc
目录号
NM-HU-250354
品系状态
活体
基因信息
基因名
Trp53
品系描述
验证数据
Fig.1 Detection of TP53 expression in spleen and thymus by RT-PCR. Spleen and thymus RNA was extracted from 6-week-old male wild-type C57BL/6 (WT)(n=1) and homozygous hTP53-R273H knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 mRNA sequencing of hTP53-R273H knockin mice.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of P53 expression in hTP53-R273H mice by WB.
The spleen tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hTP53-R273H mice, and then analyzed by western blot with anti-P53 antibody.
Abbr. HO, homozygous; WT, wild type. Note. The hTP53-R273H mice and C57BL/6 mice were i.p. injected with LPS (5mg/kg, 6h).
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