Slc17a6-IRES-Cre

品系全名

C57BL/6JSmo-Slc17a6em1(IRES-iCre)Smoc

目录号

NM-KI-200087

品系状态

活体

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基因信息

基因名
Slc17a6

品系描述

将IRES-iCre插入到小鼠Slc17a6基因终止密码子处。
应用领域:Cre工具鼠,兴奋性谷氨酸能神经元细胞体等区域; 谷氨酸转运蛋白 2 基因Vglut2,又称Slc17a6,在多脑区表达。Slc17a6-IRES-Cre与含有loxP序列的品系杂交时,Cre 介导的重组会导致后代侧翼序列的组织特异性缺失。

验证数据

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图 1 Slc17a6Cre/+; Rosa26tdTomato/+ 小鼠脑部tdTomato的表达情况。

Slc17a6Cre/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠皮层、海马和丘脑中检测到tdTomato的表达。

image.png

图 2 Slc17a6Cre/+; Rosa26tdTomato/+ 小鼠视网膜中tdTomato的表达情况。

Slc17a6Cre/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠视网膜神经节细胞层中检测到tdTomato的表达。

image.png

图 3 Slc17a6Cre/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况。TdTomato的表达可在脑的皮层、海马、纹状体和丘脑等中表达。个别细胞表达的组织有:视网膜神经节细胞层和外网层、垂体、心脏、肾、附睾、睾丸、棕色脂肪、胸腺、脾脏、胃、骨骼肌、小肠和大肠、唾液腺、胰岛。不表达的组织有:肺、肝和表皮。(更多详细信息请联系我司技术顾问。)




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