Opn4-Cre

品系全名

C57BL/6JSmo-Opn4em1(Cre-WPRE-polyA)Smoc

目录号

NM-KI-200304

品系状态

精子冻存

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基因信息

基因名
Opn4

品系描述

将Cre-WPRE-polyA插入到小鼠Opn4基因起始密码子处。 Opn4(Melanopsin)编码一种光感受性视蛋白(黑素蛋白),Opn4-Cre与含有 loxP 侧翼序列的小鼠交配,Cre 介导的重组将导致后代 Opn4阳性细胞中 floxed 序列的缺失。可用于昼夜节律调节的研究。
应用领域:Cre工具鼠,昼夜节律调节

验证数据

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图 1 Opn4Cre/+; Rosa26tdTomato/+小鼠眼部tdTomato的表达情况。

Opn4Cre/+; Rosa26tdTomato/+双转基因鼠,可在小鼠视网膜神经节细胞层(RGC)和内核层细胞中检测到tdTomato的表达。

image.png

图 2 Opn4Cre/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况。TdTomato的表达可在眼球、大脑海马区、嗅球部分细胞、大肠浆膜层、睾丸和附睾、少数肺部支气管上皮细胞、肾、胸腺、垂体部分细胞中检测到。不表达的组织有:小脑、心脏、肝脏、脾、肌肉、皮肤、胃、小肠和颌下腺。(更多详细信息请联系我司技术顾问。)



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