Tnf-KO

品系全名

C57BL/6JSmo-Tnfem1Smoc

目录号

NM-KO-00100

品系状态

活体

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基因信息

基因名
Tnf

基因曾用名

DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

NCBI ID

21926

MGI ID

104798

Ensembl ID

ENSMUSG00000024401

Pubmed

Tnf

人类同源基因

TNF

品系描述

Tnf基因位于小鼠17号染色体。设计sgRNA针对Tnf基因exon 2-3，建立该Manf基因敲除小鼠模型
应用领域：免疫相关研究；肿瘤相关研究

疾病预测

脊柱关节病
Spondyloarthropathy

近似模型的表型

MGI:3629514

参考文献

Sakkou M, Chouvardas P, Ntari L, Prados A, Moreth K, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Denis MC, Karagianni N, Kollias G, Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis. JCI Insight. 2018 Apr 5;3(7)

类风湿关节炎
Rheumatoid Arthritis

近似模型的表型

MGI:3622060

参考文献

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

类风湿关节炎
Rheumatoid Arthritis

近似模型的表型

MGI:3622062

参考文献

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

验证数据

image.png

Fig.1 Detection of TNF expression in spleen and thymus by RT-PCR. 

Wild type: only one band at 209 bp with primers F1/R1(mTnf). 

Abbr. M, DNA marker;  HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of mTnf expression in spleen and thymus by Q-PCR. 

image.png

Fig.3 Detection of mTNF-a expression in serum by ELISA (male, 8wks, n=2). 

Abbr. HO, Homozygous; WT, wild type.

Note.TNF-KO and C57BL/6 mice were i.p. injected with LPS .

发表文献 2篇

1. CD11c+CD69+ trained APCs in lungs mediate allergic asthma through increased H3K27ac
发表年份：2025 来源杂志：INTERNATIONAL IMMUNOPHARMACOLOGY

2. ZBP1-mediated apoptosis and inflammation exacerbate steatotic liver ischemia/reperfusion injury
发表年份：2024 来源杂志：JOURNAL OF CLINICAL INVESTIGATION

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