H11-CAG-LSL-mtagBFP2

品系全名

C57BL/6JSmo-lgs2em1(CAG-LSL-mTagBFP2)Smoc

目录号

NM-KI-190006

品系状态

精子冻存

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品系描述

将CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA结构定点敲入到小鼠H11位点。该品系小鼠可与Cre小鼠交配,在表达Cre的细胞或组织中表达mTagBFP2-3xFLAG。
应用领域:荧光示踪

验证数据

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Fig1.荧光检测CAG-mTagBFP2/+小鼠BFP的表达。

CAG-mTagBFP2/+小鼠由CAG-LSL-mTagBFP2/+小鼠与胚胎表达Cre的小鼠交配获得。取 CAG-mTagBFP2/+小鼠的主要脏器心、肝、脾、肺、肾、脑、胸腺等组织进行荧光检测,结果发现各组织中BFP的表达较强。A代表CAG-mTagBFP2/+小鼠各组织全标本检测结果。B代表共聚焦显微镜检测CAG-mTagBFP2/+小鼠组织切片的结果。



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