R26-CAG-LSL-Luc-tdTomato

品系全名

C57BL/6JSmo-Gt(ROSA)26Sorem1(CAG-LSL-luci-2A-tdTomato)Smoc

目录号

NM-KI-18051

品系状态

活体

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基因信息

基因名
Gt(ROSA)26Sor

品系描述

将CAG-Loxp-STOP-Loxp-luci-2A-tdTomato-wpre-polyA条件性表达结构定点敲入到小鼠Rosa26基因位点中。该品系小鼠可与Cre小鼠交配,在表达Cre的细胞或组织中表达luci荧光素酶和tdTomato荧光蛋白,用于细胞示踪。
应用领域:细胞示踪

验证数据

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图1 短曝光下活体成像系统检测出 R26-CAG-LSL-luci-tdTomato;Dppa3-Cre双阳性小鼠相比野生型和R26-CAG-LSL-luci-tdTomato 单阳性小鼠,全身呈现明显荧光信号。

图2.png


图2 长曝光下活体成像系统检测出 R26-CAG-LSL-luci-tdTomato 单阳性小鼠相比于野生型小鼠,呈现更多荧光信号,显示该小鼠在未与Cre鼠交配前Luciferase已经出现少许渗漏。

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图 3 Dppa3Cre/+; Rosa26Luc-tdTomato/+小鼠肝脏,胃,小肠和大肠中tdTomato的表达情况。

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图 4 Dppa3Cre/+; Rosa26Luc-tdTomato/+小鼠各组织中tdTomato的表达情况。TdTomato可在脑、眼、心脏、脾脏、肾脏、胰腺、胸腺、唾液腺、肺、皮肤、脊髓、睾丸和附睾中检测到。(更多详细信息请联系我司技术顾问。)



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