Itgam-IRES-hCD89

品系全名

C57BL/6Smoc-Itgamem1(IRES-hCD89-WPRE-polyA)Smoc

目录号

NM-KI-200063

品系状态

活体

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基因信息

基因名
Itgam

品系描述

将IRES-hCD89-WPRE-polyA插入到小鼠Itgam基因终止密码子处。
应用领域:IgA肾病小鼠模型

验证数据

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Fig1. Expression characterization of hCD89 in homozygous Itgam-IRES-hCD89 knockin mice in C57BL/6 background.

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Fig2. Urine protein detection of Itgam-IRES-hCD89 mice. 

The urine protein of the mice was measured by visual urine protein test strip every 3 weeks. According to the degree of color development in the reaction area, the urine protein level is divided into  negative (-), 15 mg/L (±), 30 mg/L (+), 100 mg/L (++), and 300 mg/L (+++) . The results showed that Itgam-IRES-hCD89 mice started to develop proteinuria between 9-15 weeks of age. (Data provided by partners)

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Fig3. Analysis of serum creatinine levels in Itgam-IRES-hCD89 mice. 

The serum creatinine level of the 24-week-old Itgam-IRES-hCD89 mice increased significently.(Data provided by partners)image.png

Fig4. Analysis of IgA expression distribution. IgA deposition can be detected in the glomerulus and mesangial area of the renal tubules in the Itgam-IRES-hCD89 mice, but no deposition was found in the wild-type mice. (Data provided by partners)



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