R26-CAG-LSL-RSR-tdTomato-2A-DTR

品系全名

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-RSR-tdTomato-2A-DTR)Smoc

目录号

NM-KI-190086

品系状态

活体

导出PDF

基因信息

基因名
Gt(ROSA)26Sor

品系描述

将CAG-LSL-RSR-tdTomato-2A-DTR插入到小鼠Rosa26基因中。
应用领域:荧光报告小鼠

验证数据

image.png

Fig1. Immunostaining for tdTomato on sections of multiple tissues or organs collected from ACTB-Cre;R26-LR-tdT-DTR mice (upper panel) or CAG-Dre;R26-LR-tdT-DTR mice (lower panel). 

image.png

Fig2. DTR-mediated cardiomyocyte ablation after DT administration. 

A, a schematic figure showing experimental design. B, whole-mount view of triple transgenic mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C, immunostaining for DTR and tdTomato on heart sections of triple transgenic mice. White scale bar represents100 μm.  D, immunostaining for cTnT and tdTomato on heart sections of triple transgenic mice. White scale bar represents 100 μm. E, immunostaining for Tunel and tdTomato on heart sections. White scale bar represents 100 μm.

image.png

Fig3. DTR-mediated specific ablation of Cyp2e1+ peri-central hepatocytes.

A, a cartoon figure showing the mouse’s mating strategy. B, whole-mount epifluorescence view of livers collected from mice treated with DT or PBS. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections treated with PBS. White scale bar represents 100 μm. C, central vein. E, immunostaining for CYP2E1, tdTomato, and E-cad on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm. F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison of tdTomato+ hepatocytes (PBS group). G, immunostaining for Tunel and tdTomato on liver sections from mice treated with PBS or DT. White scale barrepresents 100 μm.

image.png

Fig4. DTR-mediated specific ablation of Mfsd2a+ peri-portal hepatocytes.

A, a schematic figure showing the genetic recombination of the R26-LR-tdT-DTR allele by Mfsd2a-CreER and AAV8-TBG-Dre.  B, whole-mount fluorescent view of livers collected from mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections of mice treated with PBS.White scale bar represents100 μm. E, immunostainingfor E-cad and tdTomato on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm.  F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison with tdTomato+ hepatocytes (PBS group). n = 5.  G, immunostaining for Tunnel and tdTomato on liver sections of mice treated with PBS or DT. White scale bar represents 100 μm.

*以上数据源自:Wang H, He L, Li Y, et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo. J Biol Chem. 2022;298(6):101965. doi:10.1016/j.jbc.2022.101965



你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式,包括基因定点修饰的小鼠命名,比如:敲除、敲入、点突变等等,和随机转基因的小鼠命名。

查看