hIL6/hIL6R

品系全名

C57BL/6Smoc-Il6tm1(hIL6)Il6raem1(hIL6R)/Smoc

目录号

NM-HU-190025

品系状态

活体

导出PDF

基因信息

基因名
IL6

基因名
IL6Ra

品系描述

通过将hIL6和hIL6R小鼠交配获得该IL6和IL6R双人源化小鼠模型。双重人源化小鼠模型可用于评估潜在免疫治疗药物组合的功效。
应用领域:免疫治疗;肿瘤研究;药物筛选

验证数据

IL6-1.png

Fig1 The expression of human IL6 in liver tissue of humanized IL6/IL6R mice was measured by qPCR. The results showed that the active expression of humanized IL6 can be detected in liver tissue of homozygous humanized IL6/IL6R mice after LPS Activation. 

IL6-2.png

Fig2 Expression of human IL6 in the serum of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6 can be detected in the serum collected from homozygous mice after LPS Activation. 

IL6-3.png

Fig3 Expression of human IL6R in the serum, liver and pancreas of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6R can be detected in the serum, liver and pancreas collected from homozygous mice. 

image.png

Fig4. Detection of hIL-6R expression in MM.1S cells.

image.png

Fig5. Detection of hIL-6R expression in T cells in hIL-6/hIL-6R dKI mice.

image.png

Fig6. Detection of hIL-6R expression in Myeloid cells, T cells and activated B cells in hIL-6/hIL-6R dKI mice. Note.  Splenocytes from C57BL/6 and homozygous IL-6/hIL-6R mice were stimulated with LPS in vitro

image.png

Fig7. Detection of hIL-6R expression in brainstem in hIL-6/hIL-6R dKI mice by IHC.

image.png

Fig8. Detection of hIL-6R expression in spleen in hIL-6 /hIL-6R dKI mice by IHC.

image.png

Fig9. Detection of hIL-6R expression in lung and thymus in hIL-6 /hIL-6R dKI mice by IHC.


你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式,包括基因定点修饰的小鼠命名,比如:敲除、敲入、点突变等等,和随机转基因的小鼠命名。

查看