CAG-LSL-dCas9-SPH


品系基本信息

品系全名

C57BL/6-Tg(CAG-LSL-dCas9-SPH)Smoc

目录号

NM-TG-00025

基因名

dCas9

基因曾用名

人类同源基因

Ensembl ID

MGI ID

NCBI ID


品系描述

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).


应用领域

基因调控工具,基因激活,CRISPR

状态与订购

品系状态

活体


你也可能感兴趣

全身性基因敲除小鼠定制低至2.46万元!

目的基因随你选,定制不受限;交付标准不缩水,杜绝二次收费;更有ModelBooster技术,助你快速获得大量子代小鼠,缩短研究周期。

查看
在线咨询 请拨打400 728 0660 模型定制方案 回到顶部