hBDNF-V66M

Fig.1 Detection of BDNF expression in brain of WT C57BL/6 and HO hBDNF-V66M mice by RT-PCR (male, 8 weeks old).

Wild type: only one band at 147 bp with primers F1/R1(mBdnf);

Homozygous: only one band at 488 bp with primers F2/R2(hBDNF).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 mRNA sequencing of hBDNF-V66M knockin mice. 

Sequencing data of RT-PCR products confirmed the presence of the mutation in hBDNF-V66M knockin mice. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human BDNF (A) and mouse BDNF (B) expression in brain homogenate of WT C57BL/6 and HO hBDNF-V66M mice by ELISA (male, 8 weeks old, n=2). 

Abbr.  HO, homozygous; WT, wild type.

Note.  The ELISA kit is cross-reactived between human and mouse BDNF.