hIGF1R(2)/hTFR1

品系全名

C57BL/6JSmo-Igf1rtm(hIGF1R)Tfrctm1(hTFRC)Smoc

目录号

NM-XA-253761

品系状态

活体

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品系描述

通过hIGF1R(2)(NM-HU-230053)与hTFR1(NM-HU-215059)小鼠交配获得。

验证数据

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Fig.1 Detection of hIGF1R expression on CD45- cells in the brain of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. hIGF1R was exclusively detectable in the brain of hIGF1R(2)/hTFR1 humanized mice but not in wild-type mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-hCD221. 

Abbr. HO, homozygous; WT, wild type.

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Fig.2 Detection of hIGF1R expression on CD45- cells in the lung of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. hIGF1R was exclusively detectable in the lung of hIGF1R(2)/hTFR1 humanized mice but not in wild-type mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-hCD221. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hTFR1 expression on CD45- cells in the brain of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. hTFR1 was exclusively detectable in the brain of hIGF1R(2)/hTFR1 humanized mice but not in wild-type mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-hCD71. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig.4 Detection of hTFR1 expression on CD45- cells in the lung of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. hTFR1 was exclusively detectable in the lung of hIGF1R(2)/hTFR1 humanized mice but not in wild-type mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-hCD71. 

Abbr. HO, homozygous; WT, wild type.

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Fig.5 Detection of mTFR1 expression on CD45- cells in the brain of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. mTFR1 was exclusively detectable in the brain of wild-type mice but not in hIGF1R(2)/hTFR1 humanized mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-mCD71. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig.6 Detection of mTFR1 expression on CD45- cells in the lung of WT C57BL/6 mice and HO hIGF1R(2)/hTFR1 mice by FACS. mTFR1 was exclusively detectable in the lung of wild-type mice but not in hIGF1R(2)/hTFR1 humanized mice. CD45- cells were collected from wild-type C57BL/6 mice (male, 6-8 weeks old) and homozygous hIGF1R(2)/hTFR1 humanized mice (male, 7 weeks old), and analyzed by flow cytometry with anti-mCD71. 

Abbr. HO, homozygous; WT, wild type.

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