hANGPTL4(2)

Fig.1 Detection of ANGPTL4 expression in mammary gland and adrenal of WT C57BL/6 (female, 8-10 weeks old) mice and HO hANGPTL4 (female, 11weeks old) knockin mice by RT-PCR. 

Wild type: only one band at 198 bp with primers F1/R1(mAngptl4);

Homozygous: only one band at 132 bp with primers F2/R2(hANGPTL4).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of hANGPTL4 and mAngptl4 expression in Liver and eWAT by qPCR. (male, 7weeks old, n=2)

Relative expression represents the hANGPTL4 or mAngptl4 mRNA levels relative to the average expression in the liver of hANGPTL4(2) knockin mice. 

Abbr.  HO, homozygous; WT, wild type; eWAT, Epididymal white adipose tissue.

Fig.3 Detection of human ANGPTL4 expression in mammary gland from female mice by ELISA (n=2 for WT group and n=3 for hANGPTL4 group, 10-11-week-old ). 

Abbr.  HO, homozygous; WT, wild type.

Note. The ELISA kit is cross-reactived between human and mouse ANGPTL4.

Fig.4 Detection of human ANGPTL4 expression in Liver and eWAT from male mice by ELISA (male, 7weeks old, n=2). 

Abbr.  HO, homozygous; WT, wild type; eWAT, Epididymal white adipose tissue.

Note. The ELISA kit is cross-reactived between human and mouse ANGPTL4.