hCD3E
品系全名
C57BL/6JSmo-Cd3etm1(hCD3E) Smoc
目录号
NM-HU-00114
品系状态
活体
基因信息
基因名
CD3E
品系描述
验证数据
FACS表达验证
Fig1. FACS 检测CD3E人源化小鼠外周血T淋巴细胞中人源CD3E的表达。
结果显示： CD3E人源化杂合子小鼠外周血T淋巴细胞可以检测到人源CD3E和鼠源Cd3e的表达，且两者表达趋势类似。 CD3E人源化纯合子小鼠仅能检测到人源CD3E的活跃表达。
Fig2. CD3E人源化小鼠外周血中T 细胞亚群检测。 CD3E人源化小鼠体内CD4+/CD8+ T 细胞比例与野生型小鼠类似，Treg细胞含量表现正常。
Fig3. CD3E人源化小鼠外周血中B细胞和NK细胞检测。 CD3E人源化小鼠体内B细胞和NK细胞含量与野生型小鼠类似。
Fig4. CD3E人源化小鼠T细胞激活功能流式分析。 CD3E人源化杂合子小鼠即可以被抗鼠源CD3抗体激活，也可以被抗人源抗体激活。 CD3E人源化纯合子小鼠可以被抗人源CD3抗体激活。
Fig5. CD3E人源化小鼠T细胞激活后的细胞因子检测。从 CD3E人源化小鼠脾细胞中分离 T 细胞分别使用抗人源或鼠源 CD3 的抗体刺激。检测CD3E人源化小鼠IL-1β、TNF-α、IFN-γ、lL-4、IL-5和IL-6 的浓度。
以上数据均为南模生物与Crownbio合作完成。
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