hGCGR

Fig.1 Detection of Mouse Gcgr expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of GCGR expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.3 Detection of hGCGR expression in hGCGR mice by WB.

Abbr. M,marker; HO, homozygous; WT, wild type; PC, positive control, Hela cells.

Note.The anti-GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

Fig.4 Detection of hGCGR expression in Kidney in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

Fig.5 Detection of hGCGR expression in Liver in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.