hPD-L1

品系全名

C57BL/6JSmo-Cd274tm1(hPD-L1)Smoc

目录号

NM-HU-00062

品系状态

活体

导出PDF

基因信息

基因名
Cd274

基因曾用名

B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik

NCBI ID

60533

MGI ID

1926446

Ensembl ID

ENSMUSG00000016496

Pubmed

Cd274

人类同源基因

CD274

品系描述

通过同源重组，将小鼠基因Cd274进行人源化修饰。与此相似的品系还有hPD-L1(2)(NM-HU-190039),详细信息请咨询技术顾问。
应用领域：免疫治疗；肿瘤研究；药物筛选

验证数据

image.png

Fig1. Expression of PD-L1 in the spleen lymphocytes collected from homozygous humanized PD-L1 mice and wild-type mice is detected by FACS. The results showed that the expression of human PD-L1 can be detected in both T cells and B cells collected from the spleen of homozygous humanized PD-L1 mice. (Completed in collaboration with CrownBio)

image.png

Fig2. Immunotype in blood , spleen and bone marrow in hPD-L1 mice.  

image.png


Fig3. Immunotype in blood in hPD-L1 mice. 

image.png

Fig4. Immunotype in spleen in hPD-L1 mice. 

image.png

Fig5. Immunotype in bone marrow in hPD-L1 mice. 

image.png

Fig6. In vivo validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized PD-L1 mice. Homozygous humanized PD-L1 mice were inoculated with MC38 colon cancer cells (expressing human PDL1 rather than murine PD-L1). After the tumors grew to 100 mm3, the animals were randomly assigned into a control group and a treatment group (n=5). The results showed: The antibodies targeting human PD-L1 were associated with a very significant anti-tumor effect (TGI: tumor growth inhibition, p < 0.001), demonstrating that the humanized PD-L1 mice are a good in vivo model for validating the efficacy of antibodies targeting human PD-L1.

发表文献 10篇

1. Intratumoural vaccination via checkpoint degradation-coupled antigen presentation
发表年份：2026 来源杂志：NATURE

2. High-Affinity Decoy PD-1 Mutant Screened from an Epitope-Specific Cell Library
发表年份：2021 来源杂志：Engineering

3. Single-cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironment
发表年份：2021 来源杂志：BMC BIOLOGY

4. Dose escalation biodistribution, positron emission tomography/computed tomography imaging and dosimetry of a highly specific radionuclide-labeled non-blocking nanobody
发表年份：2021 来源杂志：EJNMMI Research

5. Therapeutic Effect of an Anti-Human Programmed Death-Ligand 1 (PD-L1) Nanobody on Polymicrobial Sepsis in Humanized Mice
发表年份：2021 来源杂志：MEDICAL SCIENCE MONITOR

6. Discovery of novel CA-4 analogs as dual inhibitors of tubulin polymerization and PD-1/PD-L1 interaction for cancer treatment
发表年份：2020 来源杂志：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

7. A Novel Bispecific Antibody with PD-L1–assisted OX40 Activation for Cancer Treatment
发表年份：2020 来源杂志：MOLECULAR CANCER THERAPEUTICS

8. Anticancer Effect of Salvia plebeia and Its Active Compound by Improving T-Cell Activity via Blockade of PD-1/PD-L1 Interaction in Humanized PD-1 Mouse Model
发表年份：2020 来源杂志：Frontiers in Immunology

9. Unripe Black Raspberry (Rubus coreanus Miquel) Extract and Its Constitute, Ellagic Acid Induces T Cell Activation and Antitumor Immunity by Blocking PD-1/PD-L1 Interaction
发表年份：2020 来源杂志：Foods

10. Distinct contribution of PD-L1 suppression by spatial expression of PD-L1 on tumor and non-tumor cells
发表年份：2018 来源杂志：Cellular & Molecular Immunology

展开

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看

【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看

Cre-lox系统介绍及使用汇总

你一定听说过Cre-lox重组系统，无论你是否直接进行过基因操作。由于Cre-lox系统具有操作简单、重组率高的优点，如今已经成为体内外遗传操作的强有力工具。利用Cre-lox系统，可以在特定细胞、组织或整个生物体，甚至在特定时间点敲除或表达某个基因，实现对特定基因的时空特异性操作，这对基因功能的研究和人类疾病动物模型的建立都具有深刻影响。

查看