hACVR1B

Fig.1 Detection of ACVR1B expression in colon by RT-PCR. Mouse Acvr1b mRNA (280 bp) was detectable only in wild-type C57BL/6 mice. Human ACVR1B mRNA (392 bp) was detectable only in homozygous hACVR1B knockin mice but not in wild-type mice. Tissue RNA was extracted from WT mice (n=3, male, 7 weeks old) and homozygous hACVR1B knockin mice (n=3, female, 9 weeks old), then cDNA libraries were synthesized by reverse transcription, followed by PCR. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of ACVR1B expression in hACVR1B knockin mice by WB. Tissues lysates were collected from wild-type C57BL/6 mice (WT, male, 7 weeks old) and homozygous hACVR1B mice (HO, female, 9-10 weeks old), and then analyzed by western blot with anti-ACVR1B antibody. 20 μg of total proteins were loaded for western blotting analysis. The antibody is cross-reactive between human and mouse ACVR1B. The positive control used is Caco-2 cells.

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control