hNGF

Fig.1 Detection of NGF expression in heart and ovary of WT C57BL/6 mice and hNGF knockin mice by RT-PCR (female, 7 weeks old). 

Wild type: only one band at 291 bp with primers F1/R1(mNgf);

Homozygous:  only one band at 280 bp with primers F2/R2(hNGF).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human NGF expression in serum from LPS-induced WT C57BL/6 mice (n=3 for each group, 1 male and 5 females, 14 weeks old) and hNGF knockin mice (n=3 for each group, female, 13 weeks old) by ELISA. 

Abbr.  HO, homozygous; WT, wild type.

Note. Mice were injected intraperitoneally with (+) or without (−) LPS (5 mg/kg), and serum was collected 2 h post-injection.