hC3/hCFB

Fig.1 Detection of human CFB(A) expression in serum of WT C57BL/6 mice and HO/HO hCFB/hC3(2) mice by ELISA. Serum were collected from wild-type C57BL/6 mice (n=3, male, 9 weeks old) and HO/HO hCFB/hC3(2) mice (n=3, male, 9 weeks old), and analyzed by ELISA with anti-hCFB ELISA kit. Human CFB was exclusively detectable in homozygous hCFB/hC3(2) knockin mice. Human CFB was exclusively detectable in homozygous hCFB/hC3(2) knockin mice. 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig.2 Detection of human C3(A) and mouse C3(B) expression in serum of WT C57BL/6 mice and HO/HO hCFB/hC3(2) mice by ELISA. Serum were collected from wild-type C57BL/6 mice (n=3, male, 9 weeks old) and HO/HO hCFB/hC3(2) mice (n=3, male, 9 weeks old), and analyzed by ELISA with anti-mC3 ELISA kit and anti-hCFB/hC3(2) ELISA kit. Human C3 was exclusively detectable in homozygous hCFB/hC3(2) knockin mice. Mouse C3 was exclusively detectable in wild-type C57BL/6 mice.  

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig.3 Detection of mouse C5(A) and mouse C5a(B) expression in serum of WT C57BL/6 mice and HO/HO hCFB/hC3(2) mice by ELISA. Serum were collected from wild-type C57BL/6 mice (n=3, male, 9 weeks old) and HO/HO hCFB/hC3(2) mice (n=3, male, 9 weeks old), and analyzed by ELISA with anti-mC5 ELISA kit and anti-mC5a ELISA kit. mC5 and mC5a was detectable in serum from both WT C57BL/6 and homozygous hCFB/hC3(2) knockin mice. mC5 levels were lower in hCFB/hC3(2) knockin mice than in WT mice. (Mean±SEM, Unpaired t-test, *p<0.05, ns: no significance). 

Abbr.  HO, homozygous; WT, wild type.