hCDCP1(2)

Fig.1 Detection of CDCP1 expression in colon by RT-PCR. Mouse Cdcp1 mRNA (194 bp) was detectable only in wild-type C57BL/6 mice. Human CDCP1 mRNA (206 bp) was detectable only in homozygous hCDCP1(2) knockin mice but not in wild-type mice. Colon RNA was extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 6-week-old homozygous hCDCP1(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of CDCP1 expression in skin, testis, heart, liver, kidney, lung, brain, colon and skeletal muscle by qPCR. Human CDCP1 mRNA was detectable only in homozygous hCDCP1(2) knockin mice but not in wild-type mice. Tissues RNA were extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 6-week-old homozygous hCDCP1(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with human CDCP1 and mouse Gapdh primers. Relative expression represents the human CDCP1 mRNA level relative to its average expression of lung in homozygous hCDCP1(2) knockin mice . 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.3 Detection of CDCP1 expression in skin, testis, heart, liver, kidney, lung, brain, colon and skeletal muscle by qPCR. Mouse Cdcp1 mRNA was detectable only in wild-type mice but not in homozygous hCDCP1(2) knockin mice. Tissues RNA were extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 6-week-old homozygous hCDCP1(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Cdcp1 and mouse Gapdh primers. Relative expression represents the mouse Cdcp1 mRNA level relative to its average expression of lung in wild-type mice . 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.4 Detection of CDCP1 expression in hCDCP1(2) mice by WB. CDCP1 was detectable in heart, liver, lung, colon, uterus and skin from both WT C57BL/6 and HO hCDCP1(2) mice, as the antibody was cross-reactived between human and mouse CDCP1. The heart, liver, lung, colon, uterus and skin lysates were collected from 7-week-old female wild-type C57BL/6 mice and 7-week-old female homozygous hCDCP1(2) mice, and then analyzed by western blot with anti-CDCP1 antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, HCT-116 cells; M, marker.