hCD19(BALB/c)

Fig1. Detection of hCD19 expression on B cells in Blood in hCD19 KI mice.

Table 1. Blood routine examination of hCD19(BALB/c) knockin mice. 

Abbr. HO, homozygous.

Values are expressed as mean±SEM.

Fig.2 Body weight and ratios of spleen, thymus and MLN to body weight of 8-week-old hCD19(BALB/c) knokcin mice. (n=7 for female WT group and n=6 for each other group, Mean±SEM, Unpaired t-test, **P<0.01,  ****P<0.0001).

Table 2. Biochemical test of serum from hCD19(BALB/c) knockin mice. 

Abbr. HO, homozygous; WT, wild type.

Values are expressed as mean±SEM.

Fig.3 Blood biochemical test of 8-week-old hCD19 (BALB/c) knokcin mice. (n=6 for all groups, Mean±SEM, Unpaired t-test, *p<0.05).

Abbr. HO, homozygous; WT, wild type.

Fig.4 Detection of myeloid cell subsets in the blood of hCD19(BALB/c) knockin mice by FACS (n=3 in all groups).

Abbr.  WT, wild type; HO, homozygous.

Fig.5 Detection of lymphocyte subsets in the blood of hCD19(BALB/c) knockin mice by FACS (n=3 in all groups).

Abbr.  WT, wild type; HO, homozygous.

Fig.6 Detection of myeloid cell subsets in the spleen of hCD19(BALB/c) knockin mice by FACS (n=3 in all groups).

Abbr.  WT, wild type; HO, homozygous.

Fig.7 Detection of lymphocyte subsets in the spleen of hCD19(BALB/c) knockin mice by FACS (n=3 in all groups).

Abbr.  WT, wild type; HO, homozygous.

Fig.8 Pathological analysis of hCD19 (BALB/c) knockin mice by H＆E staining.

There were no obvious pathological changes in these tissues. (n=3, 8-week-old) 

Fig.9 Detection of mouse IgM(A) expression in serum of WT BALB/c mice and HO hCD19(BALB/c) mice by ELISA. Serum were collected from wild-type BALB/c mice (n=6, 8 weeks old) and HO hCD19(BALB/c) mice (n=6, 8 weeks old), and analyzed by ELISA with anti-mIgM ELISA kit. Serum IgM levels were detectable and comparable between wild-type BALB/c mice and homozygous (HO) hCD19(BALB/c) knockin mice.

Abbr.  HO, homozygous; WT, wild type.

Fig.10 Detection of mouse IgG(A) expression in serum of WT BALB/c mice and HO hCD19(BALB/c) mice by ELISA.  Serum were collected from wild-type BALB/c mice (n=6, 8 weeks old) and HO hCD19(BALB/c) mice (n=6, 8 weeks old), and analyzed by ELISA with anti-mIgG ELISA kit. Compared with WT BALB/c mice, male hCD19 (BALB/c) mice exhibited a modest decrease in serum IgG concentrations, while no significant differences were observed in the female humanized mice.

Abbr.  HO, homozygous; WT, wild type.