hGCGR

品系全名

C57BL/6Smoc-Gcgrtm(hGCGR)Smoc

目录号

NM-HU-215004

品系状态

胚胎冻存

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基因信息

基因名
Cxcr5

基因曾用名

Blr1; Gpcr6; MDR15; CXC-R5; CXCR-5

染色体位置

9

NCBI ID

12145

MGI ID

103567

Ensembl ID

ENSMUSG00000047880

Pubmed

Cxcr5

人类同源基因

CXCR5

品系描述

利用同源重组，将小鼠Gcgr进行人源化修饰。
应用领域：非胰岛素依赖性Gcgr相关的高血糖素血症和糖尿病

验证数据

image.png

Fig.1 Detection of Mouse Gcgr expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of GCGR expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hGCGR expression in hGCGR mice by WB.

Abbr. M,marker; HO, homozygous; WT, wild type; PC, positive control, Hela cells.

Note.The anti-GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

image.png

Fig.4 Detection of hGCGR expression in Kidney in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

image.png

Fig.5 Detection of hGCGR expression in Liver in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

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