Wt1-CreERT2

品系全名

C57BL/6JSmo-Wt1em1(CreERT2-WPRE-pA)Smoc

目录号

NM-KI-200127

品系状态

活体

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基因信息

基因名
Wt1

基因曾用名

Wt-1; D630046I19Rik

NCBI ID

22431

MGI ID

98968

Ensembl ID

ENSMUSG00000016458

Pubmed

Wt1

人类同源基因

WT1

品系描述

将CreERT2-WPRE-pA插入到小鼠Wt1基因起始密码子处。
应用领域：Wt1在成年小鼠心脏的外膜表达，也在内脏器官的间皮中表达，可用作谱系追踪和标记Wt1表达细胞，常用于研究心脏、精子的发育及肝损伤等。

验证数据

NM-KI-200127 Wt1-CreERT2-1.png

图 1 Wt1CreERT2/+; Rosa26tdTomato/+ 小鼠心脏中tdTomato的表达情况。

经Tamoxifen诱导后，Wt1CreERT2/+; Rosa26tdTomato/+ 双转基因鼠，可在心室外膜细胞中表达tdTomato。

NM-KI-200127 Wt1-CreERT2-2.png

图 2 Wt1CreERT2/+; Rosa26tdTomato/+ 小鼠胚胎的心脏中tdTomato的表达情况。

经Tamoxifen诱导后，Wt1CreERT2/+; Rosa26tdTomato/+ 双转基因鼠，可在E10.5的心脏中表达tdTomato。

NM-KI-200127 Wt1-CreERT2-3.png

图 3 Wt1CreERT2/+; Rosa26tdTomato/+ 小鼠肝脏中tdTomato的表达情况。

经Tamoxifen诱导后，Wt1CreERT2/+; Rosa26tdTomato/+ 双转基因鼠，可在肝脏间皮细胞中表达tdTomato。

NM-KI-200127 Wt1-CreERT2-4.png

图 4 Wt1CreERT2/+; Rosa26tdTomato/+ 小鼠睾丸中tdTomato的表达情况。

经Tamoxifen诱导后，Wt1CreERT2/+; Rosa26tdTomato/+ 双转基因鼠，可在睾丸的外膜（桨膜和白膜）和实质结构的个别细胞中表达tdTomato。

NM-KI-200127 Wt1-CreERT2-5.png

图 5 Wt1CreERT2/+; Rosa26tdTomato/+ 小鼠各组织中tdTomato的表达情况。CreERT2的活性在肾小球，心脏、肝脏、脾、胃和肠（浆膜层）的外膜中经诱导后表达，脾有少量漏表达；少量胰腺和胸腺细胞诱导后表达。不表达的组织包括：脑、垂体、视网膜、肺、颌下腺、皮肤、肌肉。（更多详细信息请联系我司技术顾问。）

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