Csf2-KO

品系全名

C57BL/6JSmo-Csf2em2Smoc

目录号

NM-KO-190532

品系状态

胚胎冻存

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基因信息

基因名
CSF2

基因曾用名

CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM

NCBI ID

12981

MGI ID

1339752

Ensembl ID

ENSMUSG00000018916

Pubmed

CSF2

人类同源基因

CSF2

品系描述

敲除Csf2基因exon 1-3，建立Csf2基因敲除小鼠模型。曾有基因修饰致死报导，详情点击基因信息中的MGI ID。
应用领域：生长因子，NF-KB 信号通路，过敏、哮喘研究相关，

疾病预测

肺泡蛋白沉积症
Pulmonary Alveolar Proteinosis

近似模型的表型

MGI:2652689

参考文献

Stanley E, Lieschke GJ, Grail D, Metcalf D, Hodgson G, Gall JA, Maher DW, Cebon J, Sinickas V, Dunn AR, Granulocyte/macrophage colony-stimulating factor-deficient mice show no major perturbation of hematopoiesis but develop a characteristic pulmonary pathology. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5592-6

系统性红斑狼疮
Systemic Lupus Erythematosus

近似模型的表型

MGI:3586342
注：该品系需与Il3-KO(NM-KO-190498)交配才可能获得预期表型

参考文献

Enzler T, Gillessen S, Manis JP, Ferguson D, Fleming J, Alt FW, Mihm M, Dranoff G, Deficiencies of GM-CSF and interferon gamma link inflammation and cancer. J Exp Med. 2003 May 5;197(9):1213-9

验证数据

image.png

Fig.1 Detection of Csf2 expression in lung by RT-PCR. Lung RNA was extracted from 7-year-old male wild-type C57BL/6 (WT) and homozygous Csf2-KO mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Gapdh mRNA (123 bp) was detectable both in wild-type C57BL/6 mice and homozygous Csf2-KO mice. Mouse Csf2 mRNA (146 bp/138 bp) was detectable only in wild-type mice but not in homozygous Csf2-KO mice. 

Abbr. M, marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of Csf2 expression in lung by qPCR. Lung RNA were extracted from 7-year-old male wild-type C57BL/6 (WT) and homozygous Csf2-KO mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Csf2 and mouse Gapdh primers. Relative expression represents the mouse Csf2 mRNA level relative to its average expression in homozygous Csf2-KO mice. Mouse Csf2 mRNA was detectable only in wild-type mice but not in homozygous Csf2-KO mice.

Abbr. M, marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of mouse CSF2 expression in serum of WT C57BL/6 mice and HO CSF2-KO mice by ELISA. Serum were collected from wild-type C57BL/6 mice (n=3, male, 11 weeks old) and HO CSF2-KO mice (n=3, male, 11 weeks old), and analyzed by ELISA with anti-mCSF2 ELISA kit. Mouse CSF2 was exclusively detectable in wild-type C57BL/6 mice. 

Abbr.  HO, homozygous; WT, wild type.

Note. CSF2-KO and C57BL/6 mice were i.p. injected with LPS(5mg/kg) for 2h.

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