F9-KO

品系全名

C57BL/6JSmo-F9em1Smoc

目录号

NM-KO-18046

品系状态

胚胎冻存

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基因信息

基因名
F9

基因曾用名

Cf9; Cf-9; AW111646

染色体位置

X

NCBI ID

14071

MGI ID

88384

Ensembl ID

ENSMUSG00000031138

Pubmed

F9

人类同源基因

F9

品系描述

F9基因位于X染色体上，通过针对小鼠F9基因exon8设计gRNAs，获得F9基因敲除小鼠模型。F9缺失会导致小鼠发生凝血功能障碍。F9基因敲除纯合子小鼠可正常发育、存活、繁育。饲养时需注意同笼小鼠间争斗可能导致小鼠内出血甚至死亡，剪尾后必须采取及时的止血措施如烧灼尾部切口，防止敲除纯合子小鼠失血和死亡。该模型是研究凝血功能障碍、F9基因功能及基因治疗方法的有力模型。通过敲除F9基因exon 8，建立F9-KO小鼠模型。与此相似的品系还有F9-KO(2)(NM-KO-200607)，敲除区域为exon 1-8。曾有基因修饰致死报导，详情点击基因信息中的MGI ID。
应用领域：F9因子缺失相关凝血机制研究和药物试验

疾病预测

血友病
Hemophilia

近似模型的表型

MGI:2175873

参考文献

Wang L, Zoppe M, Hackeng TM, Griffin JH, Lee KF, Verma IM, A factor IX-deficient mouse model for hemophilia B gene therapy. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11563-6

验证数据

f9-7.png

Fig1 F9构建策略图。（数据来源：Hum Genet. 2017;136(7):875-883. ）

f9-8.png

Fig2 F9 mRNA在WT和KO小鼠品系肝组织中的表达。（数据来源：Hum Genet. 2017;136(7):875-883. ）

发表文献 1篇

1. CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice
发表年份：2017-05-15 来源杂志：Springer Link

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