hLILRB4

品系全名

C57BL/6Smoc-Lilrb4atm1(hLILRB4 )Smoc

目录号

NM-HU-210024

品系状态

活体

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基因信息

基因名
Lilrb4a

品系描述

利用CRISPR基因编辑技术,将小鼠Lilrb4a基因全部或者部分替换为人源LILRB4 。从而表达人或人鼠嵌合LILRB4 蛋白,取代小鼠内源Lilrb4a蛋白的表达。
应用领域:免疫治疗;药物筛选

验证数据

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Fig.1 Expression analysis of hLILRB4 on myeloid cells in blood of hLILRB4 humanized mice.  

Leukocytes were collected from blood of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.

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Fig.2 Expression analysis of hLILRB4 in spleen of hLILRB4 humanized mice.

Leukocytes were collected from spleen of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.

image.png

Fig.3 Expression analysis of hLILRB4 in bone marrow (BM) of hLILRB4 humanized mice. 

Leukocytes were collected from BM of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.


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