hSORT1

利用同源重组，将小鼠Sort1基因进行人源化修饰。

Fig1. Detection of SORT1 expression in lung and colon by RT-PCR. 

Wild type: only one band at 205 bp with primers F1/R1(mSort1);

Heterozygous: one band at 205 bp with primers F1/R1(mSort1) and one band at 193 bp with primers F2/R2 (hSORT1). 

Abbr.. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of human SORT1 expression in lung in hSORT1 knockin mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note: The anti-human SORT1 antibody cross-reacted with mouse SORT1 and humanized SORT1.