Igkj-KO(BALB/c)

Fig.1 FACS panel 1 designed for detecting T cells, B cells and NK cells in Igkj-KO (BALB/c)(21-week-old, male) and WT mice(21-week-old, male).

Fig.2 FACS panel 2 designed for detecting B cells in Igkj-KO (BALB/c)(21-week-old, male) and WT mice(21-week-old, male).

Fig.3 Spleen size and weight of WT BALB/c mice and Igkj-KO(BALB/c) mice. Compared with wild-type mice, spleen weight in HO Igkj-KO mice was reduced.

Abbr. HO, homozygous; WT, wild type.

Fig.4 Detection of the frequency of immune cell subsets in the blood and spleen by flow cytometry. In the peripheral blood and spleen, compared with WT mice, the proportion of B cells within CD45+ cells was reduced in HO Igkj-KO mice, with concomitant increases in T cell, NK cell and myeloid cell proportions.

Abbr. HO, homozygous; WT, wild type.

Fig.5 Detection of the frequency of B cell subsets in the blood and spleen by flow cytometry. Most B220+ cells were CD19+ cells in both WT and in HO Igkj-KO mice. In peripheral blood and spleen, Igkj-KO mice displayed reduced proportions of IgD^highIgM^low cells and elevated proportions of IgD^low/negativeIgM^high and IgD-IgM- cells compared with WT mice.

Abbr. HO, homozygous; WT, wild type.

Fig.6 Detection of mouse IgG expression in serum of WT BALB/c mice and Igkj-KO(BALB/c) mice by ELISA. Igkj-KO mice displayed reduced serum mIgG level compared with WT mice. Serum were collected from wild-type BALB/c mice (n=2, 21 weeks old) and Igkj-KO(BALB/c) mice (n=2, 21 weeks old), and analyzed by ELISA. 

Abbr. HO, homozygous; WT, wild type.

Fig.7 Detection of mIgG(kappa) and mIgG(lambda) expression in serum of WT BALB/c mice and Igkj-KO(BALB/c) mice by ELISA. Igkj-KO mice exhibited decreased mIgG(kappa) but elevated mIgG(lambda) in serum. Serum were collected from wild-type BALB/c mice (n=2, 21 weeks old) and Igkj-KO(BALB/c) mice (n=2, 21 weeks old), and analyzed by ELISA.

Abbr. HO, homozygous; WT, wild type