Siglece-KO
品系全名
C57BL/6Smoc-Sigleceem1Smoc
目录号
NM-KO-200483
品系状态
精子冻存
基因信息
基因名
Siglece
品系描述
敲除Siglece基因exon 2-5，建立Siglece基因敲除小鼠模型。
应用领域：治疗性抗体靶点
*使用本品系发表的文献需注明: Siglece-KO mice (Cat. NO. NM-KO-200483) were purchased from Shanghai Model Organisms Center, Inc..
验证数据
Fig.1 Detection of siglece expression in spleen by RT-PCR.
Wild type: one band at 392 bp with primers F1/R1(mSiglece) and one band at 366 bp with primers F2/R2(mSiglece).
Abbr. M, DNA marker; WT, wild type； HO, homozygous.
Fig.2 Detection of siglece expression in spleen by FACS.
WT mice highly expressed Siglec-E on neutrophils, with less expression on other myeloid cells, but barely on lymphocytes, whereases Siglece-KO mice lacked expression of Siglec-E on all these immune cells.
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