Cgas-KO

通过敲除Cgas基因exon2-3，建立Cgas基因敲除小鼠模型。

Fig.1 Detection of Cgas expression in the thymus and bladder by RT-PCR. RNA expression of Cgas in Cgas-KO mice by RT-PCR.Wild type: one band at 177 bp with primers F1/R1(mCgas) and one band at 178 bp with primers F/R(mGapdh).Homozygous: one band at 178 bp with primers F/R(mGapdh).

 Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of Cgas expression in thymus and bladder by qPCR. RNA expression of Cgas in Cgas-KO mice by qPCR.Mice in LPS group were injected intraperitoneally with a single dose of LPS (5mg/kg) and sacrificed 2 h after administration. Relative expression represents Cgas mRNA levels relative to its average expression in WT mice in control group. (n=2, female, 6-week-old) 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.3 Detection of Ifnβ1 expression in thymus of the LPS-stimulated Cgas-KO mice by qPCR.mRNA expression of Ifnβ1 in thymus of Cgas-KO mice by qPCR.Relative expression represents Ifnβ1 mRNA levels relative to its average expression in WT mice in control group. (n=2, female, 6-week-old) 

Abbr. HO, homozygous; WT, wild type.

Fig.4 Detection of Ifnα2 expression in thymus of the LPS-stimulated Cgas-KO mice by qPCR.RNA expression of Ifnα2 in Cgas-KO mice by qPCR.Relative expression represents Ifnα2  mRNA levels relative to its average expression in WT mice in control group. (n=2, female, 6-week-old) 

Abbr. HO, homozygous; WT, wild type.

Fig.5 Detection of mouse cGAS expression in Cgas-KO mice by WB.Expression characterization of mouse cGAS in Cgas-KO mice by WB.Mice in LPS group were injected intraperitoneally with a single dose of LPS (5mg/kg) and sacrificed 2 h after administration. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, NIH/3T3 cells.