C1s2/C1s1-KO

品系全名

C57BL/6JSmo-C1s1em1C1s2em2Smoc

目录号

NM-KO-233484

品系状态

精子冻存

导出PDF

基因信息

基因名
C1s2

品系描述

在C1s1-KO品系基础上,通过敲除C1s2基因exon 2-11,建立C1s2(-371bp)基因敲除小鼠模型。

鉴定报告

下载

验证数据

image.png

Fig.1 Detection of C1s1 expression in bladder by RT-PCR. 

Wild type: only one band at 197 bp with primers F1/R1(mC1s1). The results showed that mouse C1s1 were verified to be knocked out at the RNA level.

Abbr. M, DNA marker;  HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of C1s2 expression in bladder by RT-PCR. 

Wild type: only one band at 318 bp with primers F2/R2(mC1s2).  The results showed that mouse C1s2 were verified to be knocked out at the RNA level.

Abbr. M, DNA marker;  HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of classical complement pathway activity in 6-week-old female WT C57BL /6 and HO C1s2/C1s1-KO mice by ELISA (Hycultbiotech, HIT420).


你也可能感兴趣

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

查看

Workshop:Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

查看

Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

查看