Lgr5-2A-DreERT2

品系全名

C57BL/6Smoc-Lgr5em1(2A-DreERT2)Smoc

目录号

NM-KI-200178

品系状态

精子冻存

导出PDF

基因信息

基因名
Lgr5

基因曾用名

FEX; Gpr49

NCBI ID

14160

MGI ID

1341817

Ensembl ID

ENSMUSG00000020140

Pubmed

Lgr5

人类同源基因

LGR5

品系描述

将2A-DreERT2插入到小鼠Lgr5基因终止密码子处。
应用领域：Dre工具鼠

验证数据

image.png

Fig1. Dre mediated recombination in liver of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse.

Hepatocyte expressing tdTomato(red) after tamoxifen induction of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse. (see the reference). 

image.png

Fig2. Dre mediated recombination in colon of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse.

Lgr5-2A-DreERT2 43T,46.jpg

Fig3. Detection of tdTomato(red) in various tissues of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse. 

Tissues expressing tdTomato after tamoxifen induction included the large intestine, small intestine, liver, stomach, and skin. In contrast, no significant fluorescent expression was observed in the brain, spinal cord, esophagus, muscle, vagina, or reproductive system of the mice following tamoxifen induction. (38-days old, female) (For more detailed information please contact our technical advisor.) 


发表文献

A Suite of New Dre-recombinase Drivers Markedly Expands the Ability to Perform Intersectional Genetic Targeting
来源杂志：CELL STEM CELL

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看