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为您找到相关结果约751个
小鼠

Paqr9-KO

品系名：B6;129S-Paqr9tm1Smoc
品系状态：胚胎冻存 | 目录号：NM-KO-00062

The entire exon was replaced by Neo cassette.

小鼠

Mir125a-KO

品系名：B6;129S-Mir125atm1Smoc
品系状态：胚胎冻存 | 目录号：NM-KO-00040

The entire exon was replaced by an Frt-Neo-Frt cassette.

小鼠

Anp32b-Flox

品系名：B6;129S-Anp32btm1(flox)Smoc
品系状态：胚胎冻存 | 目录号：NM-CKO-00043

These Anp32b flox mice possess loxP sites flanking exon 4 of the targeted Anp32b gene. Mice homozygous for a knock-out allele exhibit partial perinatal lethality associated with failure to initiate breathing, craniofacial defects, enlarged brain ventricl

小鼠

R26-CAG-LSL-PyMT-IRES-Luc-2A-EGFP

品系名：C57BL/6JSmo-Gt(ROSA)26Sorem1(CAG-LSL-PyMT-IRES-Luc-2A-EGFP-Wpre-polyA)Smoc
品系状态：胚胎冻存 | 目录号：NM-KI-190111

利用CRISPR基因编辑技术，将CAG-LSL-PyMT-IRES-Luc-2A-EGFP-Wpre-pA条件性表达框插入到小鼠Rosa26基因intron1中，建立条件性PyMT [the Polyoma Virus middle T antigen (PyVT)] 基因过表达小鼠。当与组织特异性Cre工具鼠交配后，可在特定细胞类型或组织中表达PyMT基因，同时带有荧光素酶和EGFP荧光表达。例如，可与MMTV-Cre交配，建立乳腺肿瘤自发和转移小鼠模型。

小鼠

Rps4l-Flox(AI)

品系名：C57BL/6JSmo-Rps4lem2(flox)Smoc
品系状态：精子冻存 | 目录号：NM-CKO-242022

通过在Rps4l基因exon 1敲入人工内含子。该flox小鼠可与组织特异性Cre工具鼠交配，获得在特定细胞类型或组织中敲除Rps4l基因的小鼠模型。构建策略参考文献CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice。

小鼠

CAG-LSL-dCas9-SPH-Tg

品系名：C57BL/6JSmo-Tg(CAG-LSL-dCas9-SPH)Smoc
品系状态：活体 | 目录号：NM-TG-00025
发表文献：1篇

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *该品系为客户捐赠品系，捐赠者发表文献荧光观察采用免疫荧光的方法，JAX关于该品系描述也明确无法直接观察到荧光。

小鼠

Col1a1-tetO-EML4-ALK

品系名：C57BL/6JSmo-Col1a1tm1(TetO-EML4-ALK)Smoc
品系状态：精子冻存 | 目录号：DM-KI-251860

将人源的EML4-ALK基因克隆成TetO-EML4-ALK后，原核注射FVB受精卵，插入到小鼠Col1a1基因处。该品系由暨南大学陈良院士课题组组捐赠。发表任何研究论文或其他形式的文献应引用“Zhou Q, Yang T, Yu X, et al. Lanatoside C activates the E3 ligase STUB1 to inhibit FOXP3 transcriptional activity and promote antitumor immunity. EMBO Mol Med. 2025;17(3):563-588. doi:10.1038/s44321-025-00200-y”

小鼠

Themis-KO

品系名：C57BL/6JSmo-Themisem1Smoc
品系状态：胚胎冻存 | 目录号：NM-KO-190161

敲除Themis基因的exon 4 区域，获得Themis基因敲除小鼠模型。

小鼠

Themis-Flox

品系名：C57BL/6JSmo-Themisem1(flox)Smoc
品系状态：精子冻存 | 目录号：NM-CKO-205214

在Themis基因exon 4两侧分别插入loxP位点。该flox小鼠可与组织特异性Cre工具鼠交配，获得在特定细胞类型或组织中敲除Themis基因的小鼠模型。

小鼠

Them6-KO

品系名：C57BL/6JSmo-Them6em1Smoc
品系状态：精子冻存 | 目录号：NM-KO-205698

敲除Them6基因exon 1，建立Them6基因敲除小鼠模型。

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