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为您找到相关结果约1,349个
小鼠

R26-CAG-LSL-GiDREADD(hM4Di)-2A-mCitrine

品系名:C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-HA-GiDREADD(hM4Di)-2A-mCitrine-WPRE-pA)Smoc
品系状态:活体 | 目录号:NM-KI-190093
验证数据:有

将CAG-LSL-HA-GiDREADD(hM4Di)-2A-mCitrine-WPRE-pA插入到小鼠Rosa26基因中。

小鼠

H11-CAG-LSL-Myc*T58A-IRES-Luc/Trp53-Flox

品系名:C57BL/6Smoc-Igs2em1(CAG-LSL-Myc*T58A-IRES)Trp53tm2(flox)Smoc
品系状态:在研 | 目录号:NM-XA-253651

在Trp53-Flox(NM-CKO-18005)基础上将CAG-LSL-Kozak-Myc*T58A-IRES-Luciferase-WPRE-pA插入到小鼠Igs2位点,获得H11-CAG-LSL-Myc*T58A-IRES-Luc/Trp53-Flox双基因修饰小鼠。

小鼠

Il6st-Y757F/V760A

品系名:C57BL/6Smoc-Il6stem(Y757F-V760A)Smoc
品系状态:活体 | 目录号:NM-KI-220045

将小鼠Il6st第757位Y替换为F,第760位V替换为A,建立该Il6st点突变小鼠模型。

小鼠

Anp32b-Flox

品系名:B6;129S-Anp32btm1(flox)Smoc
品系状态:胚胎冻存 | 目录号:NM-CKO-00043

These Anp32b flox mice possess loxP sites flanking exon 4 of the targeted Anp32b gene. Mice homozygous for a knock-out allele exhibit partial perinatal lethality associated with failure to initiate breathing, craniofacial defects, enlarged brain ventricl

细胞

MC38-hDLL3

品系名:MC38-hDLL3
品系状态 : 成瘤性验证完成 |  目录号:NMC-230086
验证数据:有

DLL3基因人源化的MC38小鼠结肠癌细胞系。该细胞系仅用于平台技术服务,不对外销售或转让。

小鼠

Xpc-Flox

品系名:C57BL/6Smoc-Xpcem1(flox)Smoc
品系状态:活体 | 目录号:NM-CKO-250913

通过在Xpc基因exon 3两侧分别插入loxP位点。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Xpc基因的小鼠模型。

小鼠

Pnpla3-KO

品系名:B6;129S-Pnpla3tm1Smoc
品系状态:胚胎冻存 | 目录号:NM-KO-00051

基因敲除纯合子小鼠表现出正常的能量、葡萄糖、脂肪代谢。Mice homozygous for a knock-out allele exhibit normal energy, glucose, and lipid homeostasis.

小鼠

Rps4l-Flox(AI)

品系名:C57BL/6Smoc-Rps4lem2(flox)Smoc
品系状态:活体 | 目录号:NM-CKO-242022

通过在Rps4l基因exon 1敲入人工内含子。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Rps4l基因的小鼠模型。构建策略参考文献CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice。

小鼠

CAG-LSL-dCas9-SPH-Tg

品系名:C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
品系状态:活体 | 目录号:NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *该品系为客户捐赠品系,捐赠者发表文献荧光观察采用免疫荧光的方法,JAX关于该品系描述也明确无法直接观察到荧光。

小鼠

Mid2-Flox

品系名:C57BL/6Smoc-Mid2em1(flox)Smoc
品系状态:活体 | 目录号:NM-CKO-242742

通过在Mid2基因exon 3-4两侧分别插入loxP位点。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Mid2基因的小鼠模型。

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