hIL4/hIL4R

品系全名

C57BL/6Smoc-Il4tm3(hIL4)Il4ratm1(hIL4R)Smoc

目录号

NM-HU-2000106

品系状态

活体

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基因信息

基因名
IL4

基因名
IL4Ra

基因名
Il4

品系描述

通过两种单靶点人源化小鼠交配获得。
应用领域:免疫治疗;肿瘤研究;药物筛选

验证数据

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Fig1. Analysis of hIL4 expression in serum by ELISA. The homozygous KI mice express hIL4 in serum after treatment with concanavalin.

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Fig2. Analysis of hIL4R expression in the spleen by FACS. The homozygous KI mice express hIL4R in the spleen, and the WT mice only express mIL4R.

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Fig3. Evaluation of an Anti-huIL4Ra mAb in the OXA-Induced Atopic Dermatitis Model in hIL4/hIL4Ra dKI Mice. Both dupilumab and Dexamethasone ameliorate overall atopic dermatitis activity in OXA - challenged ears, but only Dexamethasone worked in back skin. (In cooperation with CrownBio)

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Fig4. Dupilumab significantly mitigates inflammatory cell infiltration in lesioned ear. (In cooperation with CrownBio) 

Note: Inflammatory cell infiltration is indicated by black arrows, 10X images

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Fig5. Low dose dupilumab significantly mitigates inflammatory cell infiltration in lesioned skin.  (In cooperation with CrownBio) 

Note: Inflammatory cell infiltration is indicated by black arrows, 10X images

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Fig6. Dexamethasone and dupilumab treatments are well tolerated. Both significantly reduced IgE levels in serum but not spleen weight. (In cooperation with CrownBio) 

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.

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Fig7. Repeated OXA-induced Th2 cytokine production (hIL-4 elevation) and dupilumab treatment significantly reduces IL-6 and KC/GRO levels in lesioned ear. (In cooperation with CrownBio) 

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.

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Fig8. Repeated OXA-induced Th2 cytokine production (hIL-4 elevation) and dupilumab treatment significantly reduces IL-5 and IL-2 levels in lesioned skin. (In cooperation with CrownBio) 

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.



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