hC3

品系全名

C57BL/6Smoc-C3em1(hC3)/Smoc

目录号

NM-HU-2000079

品系状态

活体

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基因信息

基因名
C3

品系描述

通过同源重组,将小鼠C3基因进行人源化修饰。

应用领域:免疫治疗;药物筛选

验证数据

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Fig1. Detection of C3 expression in liver by RT-PCR. 

Wild type: only one band at 359 bp with primers F1/R1(mC3);Heterozygous: one band at 359 bp with primers F1/R1(mC3) and one band at 357 bp with primers F2/R2(hC3); 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hC3 expression in serum by ELISA. 

Abbr. Het, heterozygous; WT, wild type. Note: Data from the collaborator.

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Fig3. Detection of hC3 expression in serum by ELISA. 

Note: The human Complement C3 ELISA Kit (ab108823) specifically recognized humanized C3.

Abbr. HO, homozygous; WT, wild type.

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Fig4. The C3hu/hu mice showed inflammation in the portal region and bile duct hyperplasia (100× and 400×).

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Fig5. The C3hu/hu mice showed glomerular sclerosis, glomerular atrophy and inflammation of the capillary tufts area (100× and 400×).

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Fig6. Body Weight of C3hu/hu mice (n=12/group).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

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Fig7. Survival curve of C3hu/hu mouse (n=12/group).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

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Fig8. Serum Biochemistry of C3hu/hu mice (n=5/group).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.image.png

Fig9. Serum Biochemistry of C3hu/hu mice (n=5/group, a different biochemical analyzer was used than in Fig8).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

image.pngFig10. Urinalysis of C3hu/hu mice.

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.


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