hCFB

品系全名

C57BL/6Smoc-Cfbtm1(hCFB)/Smoc

目录号

NM-HU-2000078

品系状态

活体

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基因信息

基因名

品系描述

通过同源重组,将小鼠Cfb基因进行人源化修饰。
应用领域:免疫治疗;药物筛选

验证数据

image.png

Fig1. Detection of CFB expression in liver by RT-PCR. 

Wild type: only one band at 441 bp with primers F1/R1(mCfb);

Homozygous: only one band at 442 bp with primers F2/R2(hCFB); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig2. Detection of human and mouse CFB expression in serum by ELISA (n=2). 

Abbr. HO, homozygous; WT, wild type.

Note: The mouse CFB ELISA kit cross-reacted with mCFB and humanized CFB.

image.png

Fig3. Detection of liver CFB mRNA remaining at Day 7 (A) and serum protein level (B) after single dose of test drug (n=3/group). (Data from a collaborator)

image.pngFig4. H&E Staining of kidney after single dose of test drug.(Data from a collaborator)

A: normal glomeruli; B: normal interstitial; C: mesangial cell proliferation, crescent body, with inflammation (black arrow); D: renal interstitial inflammation, monocyte aggregation (white arrow); E&F: mild interstitial inflammation in some areas (green arrow), mostly normal.


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