hIL2RA

品系全名

C57BL/6Smoc-Il2ratm1(hIL2RA)Smoc

目录号

NM-HU-190064

品系状态

活体

导出PDF

基因信息

基因名
IL2RA

基因名
Il2ra

品系描述

通过同源重组,将小鼠Il2ra基因进行人源化修饰。
应用领域:免疫治疗;肿瘤研究;药物筛选

验证数据

image.png

Fig1. Detection of IL2RA expression by RT-PCR. 

Wild type: only one band at 203 bp with primers F2/R2(mIl2ra);

Homozygous: only one band at 251 bp with primers F1/R1(hIL2RA); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.image.png

Fig2. Development and validation of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) Targeting strategy for hIL2RA knokcinmice development; (B)hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice; (C)hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice. 

image.png

Fig3. Characterization of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse; (B)hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse. 

image.png

Fig4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Completed in collaboration with CrownBio)

(A)Tumor growth curves upon treatment; (B) Body weight change upon treatment. 


你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式,包括基因定点修饰的小鼠命名,比如:敲除、敲入、点突变等等,和随机转基因的小鼠命名。

查看