Drd1-2A-CreERT2

品系全名

C57BL/6JSmo-Drd1em1(2A-CreERT2-WPRE-pA)Smoc

目录号

NM-KI-18016

品系状态

精子冻存

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基因信息

基因名
Drd1

品系描述

也叫D1-CreERT2。将2A-CreERT2-WPRE-pA共表达结构插入到小鼠内源Drd1a基因终止密码子前,建立Drd1a-CreERT2基因敲入工具鼠模型。诱导型CreERT2受内源Drd1a启动子驱动,在胚胎及成年小鼠多巴胺能神经元中表达,包括纹状体,伏隔核,嗅结节和前额皮质等多巴胺能系统的主要投射区域。
应用领域:多巴胺能神经元Cre工具鼠

验证数据

验证数据-Drd1a-CreERT2-图片1.png

Figure 1:Drd1a-CreERT2; Rosa26-tdTomato小鼠大脑中的tdTomato表达情况。

Drd1a-CreERT2; Rosa26-tdTomato 双转基因鼠,在大脑中有tdTomato的表达,tdTomato单转基因小鼠的大脑中没有tdTomato表达,证明Drd1a-CreERT2小鼠可以成为实现Drd1a阳性细胞特异性敲除或表达的工具鼠。

验证数据-Drd1a-CreERT2-图片2.png

Figure 2:Drd1a-CreERT2; Rosa26-tdTomato 小鼠CPU(纹状体)中的tdTomato表达情况。

Drd1a-CreERT2; Rosa26-tdTomato双转基因鼠,可在大脑的多巴胺能投射系统CPU区域表达tdTomato;tdTomato单转基因小鼠的CPU没有tdTomato表达。证明Drd1a-CreERT2小鼠可以成为实现Drd1a阳性细胞特异性敲除或表达的工具鼠。


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