Anxa10-2A-CreERT2

品系全名

C57BL/6Smoc-Anxa10em1(2A-CreERT2-Wpre-pA)Smoc

目录号

NM-KI-200312

品系状态

精子冻存

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基因信息

基因名
Anxa10

基因曾用名

-

NCBI ID

26359

MGI ID

1347090

Ensembl ID

ENSMUSG00000031635

Pubmed

Anxa10

人类同源基因

ANXA10

品系描述

2A-CreERT2-Wpre-pA expression cassette was knocked into the Anxa10 gene stop codon site.
应用领域：These mice express tamoxifen induced cre recombinase from the Anxa10 locus. By mating the reporter mice with CreERT2-expressing mice, reporter gene expression can be detected in gastric mucosa epithelial cells after tamoxifen treatment. And the mice are useful for generating conditional mutations in gastric mucosa epithelial cells. This strain may useful for in the research of gastric carcinoma.

验证数据

Anxa10-2A-CreERT2-1.png

Fig.1 CreERT2-mediated recombination in the stomach of Anxa10CreERT2/+ ; Rosa26tdTomato/+ mouse. 

TdTomato(red) expression can be detected in the gastric mucosal epithelial cell of Anxa10CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

Anxa10-2A-CreERT2-2.png

Fig.2 Detection of tdTomato (red) in various tissues of Anxa10CreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. 

CreERT2-mediated recombination in the gastric mucosal cells and hair follicle cells can be induced by tamoxifen. A small degree of leakiness were detected in the thymus prior to tamoxifen exposure. Besides, tdtomato expression can not be detected in the brain, eyes, heart, lung, liver, submandibular gland, kidney, ovary, uterus, intestine, colon, spleen, brown fat, and muscle. (For more detailed information please contact our technical advisor.)

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